Hel-FL or Hel-Δ3B coexpression with FOXP3 mediates different gene transcription of Treg signature genes in CD4⁺and CD8⁺eTregs. Heat maps comparing expression of Treg signature genes that are upregulated in Tregs (TREG UP) (A) or downregulated in Tregs (TREG DOWN) (B) compared with Tconv. Each heat map shows differential expression of genes in each of the 3 donors for FOXP3 vs FOXP3⁺Hel-FL (FL, donors FL1-FL3) and FOXP3 vs FOXP3⁺Hel-Δ3B (Δ3B, donors Δ3B1-Δ3B1) in both CD4⁺ and CD8⁺ eTregs as indicated. We first identified the subset of genes that had a nominal, uncorrected P < .05 in each indicated eTreg comparison based on change in expression of the TREG UP and TREG DOWN genes. Indicated P values are based on average change in gene expression for each eTreg comparison. For each cell in the heat map, the difference of cpm values between 2 strains of cell for 1 subject was calculated and divided by the average cpm value of that gene in all 3 subjects.