The effect of CCT241736 on cell viability and induction of apoptosis and biomarker modulation of primary AML cells. (A-B) Primary cells from AML samples with FLT3 WT and FLT3-ITD were cocultured with MS-5 and treated with either DMSO control or CCT241736 at indicated doses. (A) Three days after treatment, cells were harvested, washed, and stained with annexin-V/DAPI. (B) Viable cells were counted using a Luna-II Automated Cell Counter. (C-E) Primary AML samples AML-2 (FLT3 WT) and AML-6 (FLT3-ITD) were treated with 1 µM CCT241736 and DMSO for 4 hours, the relative levels of P-STAT5 vs total STAT5 (C), and P-HH3 vs total HH3 (D) were analyzed by immunoblotting using specific antibodies (E). All experiments were performed in triplicate, and results were shown as mean ± SEM. *P < .01 compared with control.