Changes in serum EGF and T cell subsets. (A) Plasma EGF rose significantly at 6 hours post-uhCG dose in first-line patients (blue) but not in second-line patients (orange). (B) The Treg to Tcon ratio was higher at day 7 compared with baseline. Representative Treg scatterplots from a patient at baseline (C) and day 7 (D) show an elevated proportion of CD4⁺ CD25⁺ FoxP3⁺ CD127^– Tregs. Data are presented as mean ± SEM. *P < .05 compared with baseline.