Quantitative representation of CH clones at different levels of the hematopoietic hierarchy. (A) Sanger sequencing electropherograms exemplifying detection of missense, nonsense, and frameshift mutations in individual CFUs from PB CD34⁺ cells. (B) Correlation between the percentage of detected mutation-positive CFUs by Sanger and the percentage of predicted mutation-positive CFUs based on VAFs. (C) VAFs in granulocytes, monocytes, B cells, and T cells in donors and recipients shown as a heatmap. (D) VAFs in granulocytes, monocytes, B cells, and T cells in donors and recipients (box and whisker plot). Wilcoxon matched-pairs signed rank test was used to calculate statistical significance.