Reduced expression of (hemi-)ITAM-containing receptors, elevated immature platelet fraction and platelet activation markers in DKO and TKO mice. (A) Median fluorescence intensity (MFI) measured in αIIbβ3⁺ cells or αIIb⁺ cells costained for the indicated proteins in blood, n = 6 mice/genotype. (B) Representative image of reticulated platelet population as measured by flow cytometry. αIIb⁺ platelets in whole blood were gated and the percentage of reticulated platelets was determined in gate P1 (in red), in dot plot diagram forward scatter (FSC) vs RNA dye (Thiazole Orange). (C) Percentage of reticulated platelets as determined in panel B. (D) Representative image of desialylated platelet population. αIIb⁺ platelets in whole blood were gated and the percentage of desialylated platelets was determined in gate P1 (red), in dot plot diagram FSC vs peanut agglutinin lectin. (E) Percentage of desialylated platelets as determined in panel D. (F) Percentage of P-selectin⁺αIIb⁺, TLT-1⁺αIIb⁺, fibrinogen⁺αIIb⁺, or Annexin V⁺αIIb⁺ cells in blood as determined by flow cytometry, n = 6 mice/genotype. *P < .05, **P < .01, ***P < .001, 1-way ANOVA with Sidak test; mean ± SD.