17R-RvD1 enhances phagocytosis of sickle RBCs and PMNs by spleen macrophages. In vitro phagocytosis of carboxyfluorescein diacetate succinimidyl ester–labeled aged RBCs (at 4°C, 18 h; A) or PMNs (37°C, 18 h; B) from AA and SS mice by spleen MΦs (F4/80⁺ cells), as assessed using flow cytometry. Data are mean ± standard deviation from 6 independent experiments. (C-D) Phagocytosis of RBCs and PMNs by spleen MΦs in vivo in vehicle- or 17R-RvD1–treated AA and SS mice undergoing H/R (left panels). Percentages of MΦs engulfing RBCs (F4/80⁺ Ter-119⁺; C) or PMNs (F4/80⁺ Ly6G⁺; D) were calculated using flow cytometry. Results are mean ± standard deviation from 3 mice per group. Representative flow cytometry dot plots and gate criteria (right panels). ^§P < .05 vs AA MΦs + vehicle, *P < .05 vs vehicle-treated cells. MFI, mean fluorescence intensity.