17R-RvD1 reduces vascular vulnerability in SCD mice during acute VOCs. (A) Immunoblot analysis, using specific antibodies against HO-1, IL-6, ET-1, VCAM-1, and TXAS-1, of isolated aorta from AA and SS mice under normoxic conditions and treated with vehicle or 17R-RvD1 (100 ng) and exposed to H/R: hypoxia (8% oxygen; 10 hours) followed by reoxygenation (21% oxygen; 3 hours) (left panel). One representative gel from 6 gels with similar results is shown. Vertical line(s) have been inserted to indicate a repositioned gel lane. Densitometric analysis of immunoblots (right panels). All data are mean ± SD (n = 6). *P < .05 vs normoxia, °P < .05 vs healthy mice (AA), ^P < .05 vs vehicle. (B) VCAM-1 expression was evaluated in aortas isolated from AA or SS mice exposed to H/R and treated with 17R-RvD1, as above (left panels). Following incubation with the indicated antibodies, fluorescence intensities of VCAM-1 staining (green channel) were quantified in digital images (2 to 6 microscopic fields per sample taken at a ×630 magnification scale) by selecting green stained vessels using the Magic Wand Tool in Adobe Photoshop. Semiquantitative analysis of the number of pixels in the selected fields is shown in a bar graph (right panel). *P < .05.