Inactivation of Krit1 or Pdcd10 increases the expression of TM in murine brain endothelial cells. (A) Quantification of TM protein from 3 independent biological replicates in Krit1^ECKO (KO) BMECs compared with Krit1^fl/fl (Flox) BMECs (SEM, n = 3). (B) Quantification of TM protein from 3 independent biological replicates in Pdcd10^ECKO (KO) BMECs compared with Pdcd10^fl/fl (Flox) BMECs (SEM, n = 3). (C) RT-qPCR analysis of Thbd mRNA in Krit1^ECKO or Pdcd10^ECKO BMECs compared with Krit1^fl/fl or Pdcd10^fl/fl BMECs (control), respectively (SEM, n = 3). Analysis of Klf2 and Klf4 mRNA by RT-qPCR in Krit1^ECKO BMECs (D) or in Pdcd10^ECKO BMECs (E) compared with Krit1^fl/fl or Pdcd10^fl/fl BMECs (control), respectively (SEM, n = 3). *P < .05, **P < .01, ***P < .001, Student t test.