Sirt-1 inhibits iTreg differentiation after allo-BMT. Two weeks after allo-BMT (B6 to BALB/c model), spleen and liver of the recipients were harvested and analyzed. (A-D) Representative dot plots and the average percentages of Foxp3, IFN-γ, IL-4, and IL-17 expressions on gated donor CD4⁺ or CD8⁺ T cells from spleen are shown. (E-H) Representative dot plots and the frequency of Foxp3 and IFN-γ⁺ cells gated on donor CD4⁺ or CD8⁺ T cells from liver are shown. (F) The absolute number of total cells recovered from liver and absolute numbers of donor CD4⁺ and CD8⁺ T cells are depicted. (I) Cytokine analyses of mice serum on day 14 are shown (n = 8 mice/group). Student t test was used for statistical analysis. *P < .05; **P < .01; ***P < .001.