HBsAg⁺DLBCLs displays a distinctive gene expression pattern. (A) HBsAg⁺ DLBCLs showed a distinctive gene expression profiling. The normalized expression levels were analyzed using the Qlucore Omics Explorer software. Significantly and differentially expressed genes (P < .05) between HBsAg⁺ (n = 24) and HBsAg⁻ (n = 84) DLBCLs were used to draw the heatmap. (B) GSEA revealed pathway alterations in HBsAg⁺ DLBCLs compared with that in HBsAg⁻ DLBCLs. The GSEA was performed with 1000 sample permutations. Enrichments were considered significant at false discovery rate q < 0.25. (C) GSEAPreranked tool was used to analyze BCL6 core transcriptional signatures (120 genes,⁴⁷  BCL6 targeted genes derived from DLBCL,⁴⁸  ZFP36L1-bound genes,⁴⁴  and FOXO1-targeted genes⁴⁹  along the preranked genes differentially expressed in HBsAg⁺ vs HBsAg⁻ DLBCLs. Genes were preranked based on the correlation between the gene expression by comparing HBsAg⁺ and HBsAg⁻ DLBCLs. The enrichment was tested with 1000 gene set permutations. Enrichments were considered significant at false discovery rate q < 0.05. ES, enrichment score; NES, normalized enrichment score.