CAR-37 T cells exhibit robust in vitro effector functions in response to CD37 positive tumor cells. (A) Cytotoxic capacity of CAR-37 T cells was measured after overnight coculture with targets. CAR T cells were cocultured at indicated E:T ratios with indicated tumor cell lines. Increasing concentration of CAR-37 and CAR-19 T cells led to specific killing, whereas no killing was observed in the control group (UTD). The cytotoxicity assay is representative of 3 independent experiments conducted with different healthy donors. Cytokine production by CAR-37H-L, CAR-19, or UTD T cells incubated with primary CLL (B) or MCL PDX (C) tumor samples. CAR T cells were incubated with target cells for 24 hours at a 1:1 E:T ratio, and culture supernatants were analyzed by Luminex assay. Data are plotted as mean ± SEM for 3 donors.