Figure 4.
Effect of rD′D3-FP on tail-clip bleeding in FVIII-ko mice and NMRI mice. (A) The effect of rVIII-SingleChain (100 IU/kg, red circles), alone or coadministered with rD′D3-FP (2.86 mg/kg, blue circles), was assessed in a tail-clip bleeding model to rule out potential negative effects of rD′D3-FP on the hemostasis restored by rVIII-SingleChain (n = 10). A vehicle control (gray circles) was tested at 16 hours postadministration (p.a.) as negative control. There was no significant effect of rD′D3-FP on the rVIII-SingleChain–induced reduction of bleeding time at 6 or 16 hours postadministration, as calculated using variance models to the logarithmically transformed data (pairwise comparisons with Welch's t test). Significant differences as compared to vehicle-treated FVIII-ko mice are marked with an asterisk (*). Graphs show mean ± SD and individual results. (B) Bleeding time in vehicle-treated NMRI mice (saline, gray circles) was compared with NMRI mice treated with high-dose rD′D3-FP (250 mg/kg, blue circles) at 15 minutes after tail clip (n = 20). High-dose rD′D3-FP significantly prolonged bleeding time at 15 minutes postadministration as calculated using an unpaired Student t test. Dotted line represents the observation period.

Effect of rD′D3-FP on tail-clip bleeding in FVIII-ko mice and NMRI mice. (A) The effect of rVIII-SingleChain (100 IU/kg, red circles), alone or coadministered with rD′D3-FP (2.86 mg/kg, blue circles), was assessed in a tail-clip bleeding model to rule out potential negative effects of rD′D3-FP on the hemostasis restored by rVIII-SingleChain (n = 10). A vehicle control (gray circles) was tested at 16 hours postadministration (p.a.) as negative control. There was no significant effect of rD′D3-FP on the rVIII-SingleChain–induced reduction of bleeding time at 6 or 16 hours postadministration, as calculated using variance models to the logarithmically transformed data (pairwise comparisons with Welch's t test). Significant differences as compared to vehicle-treated FVIII-ko mice are marked with an asterisk (*). Graphs show mean ± SD and individual results. (B) Bleeding time in vehicle-treated NMRI mice (saline, gray circles) was compared with NMRI mice treated with high-dose rD′D3-FP (250 mg/kg, blue circles) at 15 minutes after tail clip (n = 20). High-dose rD′D3-FP significantly prolonged bleeding time at 15 minutes postadministration as calculated using an unpaired Student t test. Dotted line represents the observation period.

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