ST3Gal-IV is important for reconstituting the thymus in MBMC mice. MBMC mice generated with 90% bone marrow cells from ST3Gal-IV–deficient (KO) mice (CD45.2) and 10% bone marrow cells from WT mice (CD45.1/CD45.2 heterozygous) injected into irradiated recipient mice (CD45.1) were analyzed 9 weeks after reconstitution. (A) Schematic illustration of the experimental setup. (B) Flow cytometric analysis of indicated lymphoid organs. The dot plots show the distribution of WT donor cells (CD45.1⁺CD45.2⁺), ST3Gal-IV–deficient (KO) donor cells (CD45.1⁻CD45.2⁺), and WT recipient cells (CD45.1⁺CD45.2⁻) from 1 representative mouse. Bars represent the mean ± SEM frequency of WT (blue) and ST3Gal-IV–deficient (red) cells among all donor cells from 2 independent experiments with 14 mice in total per group. ***P <. 001 by 2-tailed Student t test.