ST3Gal-IV is important for reconstituting the peripheral T-cell compartment in MBMC mice. Flow cytometric analysis of T cells (A) and B cells (B) in blood, spleens, and mLNs of MBMC mice generated as described in Figure 1. Dot plots show the frequencies of CD4⁺ and CD8⁺ T cells (A) or B cells (B) and the distribution of WT donor cells (CD45.1⁺CD45.2⁺), ST3Gal-IV–deficient (KO) donor cells (CD45.1⁻CD45.2⁺), and WT recipient cells (CD45.1⁺CD45.2⁻) within the corresponding parental gate from 1 representative mouse. Bars represent the mean ± SEM frequency of WT (blue) and ST3Gal-IV–deficient (red) cells among all donor cells from 2 independent experiments with 14 mice total per group. ***P < .001 by 2-tailed Student t test; ^###P < .001 by Mann-Whitney U test.