Clonal hierarchy of mIDH1 and co-occurring mutations. (A) Association of clinical response and mIDH1 clonal/subclonal status. mIDH1 was defined as subclonal in a sample if any comutation VAF was greater than mIDH1 VAF +5%; otherwise, it was defined as clonal. See details in the supplemental Methods. (B) Plot showing the tally of how often a co-occurring gene VAF is observed to be lower (red) or higher (blue) than mIDH1 VAF by NGS. Only genes that were mutated in at least 5% of patients are shown. Per-gene VAFs are detailed in supplemental Figure 4. For each gene X, the subset of patients with a mutation in gene X is selected (n in parentheses) and plotted, with the x-axis showing the percentage of patients where gene X VAF > mIDH1 VAF +5% and the y-axis showing the percentage of patients where gene X VAF < mIDH1 VAF −5%.