TF inhibition attenuates formation of LPS-induced pulmonary vaso-occlusions in SCD mice. The effect of anti-TF 1H1 antibody (5 mg/kg, IV) on the formation of neutrophil/platelet microemboli. 1H1 was administered 30 minutes prior to infusion with LPS (0.1 µg/kg, IV). Arterioles were imaged 2 to 2.5 hours after LPS infusion using qFILM. The mean pulmonary arteriolar vaso-occlusion (PVO) area (A) and average number of PVO per field of view (FOV) (B) were quantified in control sickle mice (black) or sickle mice treated with 1H1 (gray). n = 5 mice per group. Representative qFILM images of lungs from a control sickle mouse (C) or from a sickle mouse treated with 1H1 (D). The far left panels represent merged images of the circulation (FITC-dextran, purple), neutrophils (red), and platelets (green). Arrowheads denote the direction of blood flow, and dashed ovals and circles denote PVOs. Scale bars, 50 µm. *P < .05, 2-tailed, unpaired Student t test.