Decitabine induction of viral antigens persists after removal of drug. (A) qRT-PCR for LMP1 and Cp in cells treated with 250 nM decitabine vs vehicle control for 72 hours and then evaluated after removal of drug at the indicated time points. Data are shown as fold change in treated cells compared with vehicle control. Experiments were performed in triplicate. Error bars represent SEM. (B) Quantification of EBNA2⁺ cells by IHC in Rael xenograft tumors as indicated. Error bars represent SEM. *P < .05, **P < .01. (C) IHC for EBNA2 on Rael xenograft tumors after treatment with decitabine or vehicle at the specified time points. Mice were treated with decitabine or vehicle control and then evaluated immediately after treatment (n = 4), 4 days after discontinuation of drug (n = 4), or at the time of sacrifice due to progressive tumor (n = 8). Microscope, Olympus BX 43 microscope; camera, Jenoptik ProgResCF; software, ProgRes Mac Capture Pro, 2013. Original magnification ×600 with a 60/0.80 objective lens.