Figure 5.
Global EBV DNA hypomethylation is observed after decitabine treatment in latency I EBV+ BL. (A) EBV genome plot. Latent, lytic, noncoding RNA genes and regulatory regions are color coded as indicated. Capture regions for EpiTYPER MassARRAY methylation analysis are indicated with red numbers. Twenty-eight regions were selected across the genome (1-13 CpGs per region) representing primarily EBV gene promoters. Capture region for Methyl-Capture sequencing is also depicted. (B) Heatmap of quantitative DNA methylation levels as analyzed by EpiTYPER MassARRAY in vehicle- and decitabine-treated cells. (C) Heatmap of methylation of CpGs, n = 1022 from methyl-capture sequencing. Washout, cells treated with drug × 48 hours followed by 7 days of incubation in media without drug.

Global EBV DNA hypomethylation is observed after decitabine treatment in latency I EBV+ BL. (A) EBV genome plot. Latent, lytic, noncoding RNA genes and regulatory regions are color coded as indicated. Capture regions for EpiTYPER MassARRAY methylation analysis are indicated with red numbers. Twenty-eight regions were selected across the genome (1-13 CpGs per region) representing primarily EBV gene promoters. Capture region for Methyl-Capture sequencing is also depicted. (B) Heatmap of quantitative DNA methylation levels as analyzed by EpiTYPER MassARRAY in vehicle- and decitabine-treated cells. (C) Heatmap of methylation of CpGs, n = 1022 from methyl-capture sequencing. Washout, cells treated with drug × 48 hours followed by 7 days of incubation in media without drug.

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