Figure 2.
Altered MK morphology and increased number of bone marrow MKs in DKO mice. (A) BM MKs from WT and DKO mice were analyzed by transmission electron microscopy. Scale bars, 1 µm. (B) DMS dilation as a measure of altered DMS maturation was quantified using ImageJ software (National Institutes of Health). Values are mean ± SD (n = 3). Unpaired, 2-tailed Student t test. ***P < .001. (C) Confocal fluorescence microscopic images of femora cryosections of WT, Twf1−/−, Cof1−/−, and DKO mice (Leica TCS SP5). Scale bars, 50 µm. MKs, proplatelets, and platelets are shown by GPIX staining in green. CD105 staining (red) labels vessels. Nuclei were counterstained using DAPI (blue). (D) Quantification of BM MKs in whole femora cryosections. Values of Twf1−/−, Cof1−/−, and DKO mice were normalized to the respective WT control. Values are mean ± SD (n = 3). One-way ANOVA with Sidak correction for multiple comparisons. **P < .01.

Altered MK morphology and increased number of bone marrow MKs in DKO mice. (A) BM MKs from WT and DKO mice were analyzed by transmission electron microscopy. Scale bars, 1 µm. (B) DMS dilation as a measure of altered DMS maturation was quantified using ImageJ software (National Institutes of Health). Values are mean ± SD (n = 3). Unpaired, 2-tailed Student t test. ***P < .001. (C) Confocal fluorescence microscopic images of femora cryosections of WT, Twf1−/−, Cof1−/−, and DKO mice (Leica TCS SP5). Scale bars, 50 µm. MKs, proplatelets, and platelets are shown by GPIX staining in green. CD105 staining (red) labels vessels. Nuclei were counterstained using DAPI (blue). (D) Quantification of BM MKs in whole femora cryosections. Values of Twf1−/−, Cof1−/−, and DKO mice were normalized to the respective WT control. Values are mean ± SD (n = 3). One-way ANOVA with Sidak correction for multiple comparisons. **P < .01.

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