Poly(I:C) and KELIg immunoprophylaxis shift phagocytosis of transfused KEL RBCs away from CD8α DCs and toward inflammatory monocytes. (A) Gating strategy for splenic cell subsets. DiO-labeled KEL RBCs were transfused to animals treated with KELIg in the presence or absence of poly(I:C), and splenic cell subsets were evaluated at 3 and 16 hours posttransfusion (B-C). (D) Representative histograms for DiO RBC fluorescence patterns of splenic cell subsets, after first excluding TER119-positive RBCs on the exterior of the splenic cells, and DiO expression by the splenic cell subsets evaluated at 3 and 16 hours posttransfusion (E-F). These data are representative of 2 to 3 independent experiments with 3 mice per group per experiment (in total, 9, 9, and 9 mice were studied across 3 experiments for panels B and E; 6, 6, and 6 mice were studied across 2 experiments for panels C and F). *P < .05 between KELIg and KEL RBCs in the presence or absence of poly(I:C); error bars indicate standard deviation between mice. FSC, forward scatter; PBS, phosphate-buffered saline; pDCs, plasmacytoid dendritic cells; RPMs, red pulp macrophages.