Multiple inflammatory cytokines are induced by poly(I:C) in the setting of KELIg immunoprophylaxis. Animals were treated with KELIg immunoprophylaxis and transfused with KEL RBCs, in the presence or absence of poly(I:C). Serum cytokines were evaluated prior to transfusion and at 0.5, 1, and 16 hours after transfusion using the LEGENDplex kit. (A) IL-6, (B) MCP-1, (C) IFN-γ, and (D) TNF-α are shown. There were no differences observed in the 2 other cytokines (IL-10 and IL-12) evaluated. These data are representative of 2 independent experiments with 3 mice per group per experiment using LEGENDplex and 2 additional experiments with 3 mice per group per experiment using a different platform (in total, 12, 12, and 12 mice were studied across 4 experiments). *P < .05 between KELIg and KEL RBCs in the presence or absence of poly(I:C); error bars indicate standard deviation between mice.