Mn porphyrins improve organ cell apoptosis in TS mice in vivo. (A) Representative dual-labeling studies with GFP-labeled annexin V and PI of tissue organs: kidneys, liver, and spleen isolated from TS mice treated with vehicle, 0.1 mg/kg MnBuOE, and 1 mg/kg MnE. GFP-labeled annexin V–binding (green color), PI-staining (red color), and double-staining (yellow or brown color) regions are indicated with arrows (white, red, and yellow arrows, respectively). Scale bars, 50 μm. (B-G) Separate quantitation of GFP (B,D,F) and PI (C,E,G) staining in each organ: kidneys (B-C), liver (D-E), and spleen (F -G) collected from treated TS mice. Mn porphyrins could indeed alleviate organ damage as reflected by decreased apoptosis compared with the vehicle group. Data are presented as the mean of n = 5 random fields/organ/treatment condition ± SEM (n = 4 animals per treatment condition). *P < .05, **P < .01, ***P < .001, and ****P < .0001 vs vehicle treatment. FU, fluorescence unit.