Comparison of targeted sequencing using DNA from plasma or FFPE lymph node sections. (A) Plots compare amounts of mutation in plasma (human genome equivalents [hGE]) with VAF (%) in lymph node from FFPE tissue. Results are presented for 9 individual cases together with a combined plot of all patients. Individual RHOA mutations and other detected and mutated genes are color coded as shown. (B) Four cores were sequenced from a single lymph node. Results of the sequencing are indicated in the table with a color scale being used to indicate the VAF. Middle panel shows a hematoxylin and eosin–stained lymph node section indicating the positions of cores labeled C1 to C4. Right panel shows immunofluorescence staining of a sequential lymph node section to show heterogeneity of CD4 staining.