An overview of the CapTCR-Seq hybrid-capture method. (A) Hybrid-capture method experimental flow diagram. Fragments are colored based on whether they contain V-region targets (blue), J-region targets (red), D-regions (green), constant regions (yellow), or non-TCR coding regions (black). (B) V(D)J rearrangement and CDR3 sequence detection algorithm flow diagram. (C) Number of unique VJ pairs recovered relative to library DNA input amount for V probe panel capture of replicate libraries from a single PBMC sample (A037). (D) A037 polyclonal human β locus VJ rearrangements determined by CapTCR-seq. (E) A037 polyclonal human β locus VJ rearrangements determined by a PCR-based profiling service. (F) Subtractive comparison between CapTCR-seq and PCR-based profiling service. Red indicates relative enrichment of indicated pair by CapTCR-seq, whereas blue indicates relative enrichment of indicated pair by PCR-based profiling.