T-cell clonality across 5 cell lines and 4 primary T-cell specimens. Boxes represent individual unique VJ pairs, and box size reflects abundance in sample. Samples ordered by decreasing clonality. (A) β chain VJ rearrangements. (B) γ chain VJ rearrangements. (C) L2D8 gp100 antigen-specific β locus VJ rearrangements determined by CapTCR-seq. (D) L2D8 gp100 antigen-specific β locus VJ rearrangements determined by a PCR-based profiling service. (E) Subtractive comparison between CapTCR-seq and PCR-based profiling service. Red indicates relative enrichment of indicated pair by CapTCR-seq, whereas blue indicates relative enrichment of indicated pair by PCR-based profiling.