American Society of Hematology

Figure 4.

Acalabrutinib is a less potent inhibitor of integrin αIIbβ3activation and granule secretion than ibrutinib. Washed human platelets from healthy donors were preincubated with a range of acalabrutinib or ibrutinib concentrations and then stimulated with 1 µg/mL CRP-XL for 20 minutes. (A) P-selectin and (B) fibrinogen binding was measured by fluorescence-activated cell sorting. (C) Platelets were stimulated with 1 µg/mL CRP-XL for 3 minutes at 37°C. Phosphorylation of β3 (Y773) was measured by western blotting. Points represent mean responses relative to vehicle ± SEM. (D) Representative β3 (Y773) blots.

Acalabrutinib is a less potent inhibitor of integrin α_IIbβ₃activation and granule secretion than ibrutinib. Washed human platelets from healthy donors were preincubated with a range of acalabrutinib or ibrutinib concentrations and then stimulated with 1 µg/mL CRP-XL for 20 minutes. (A) P-selectin and (B) fibrinogen binding was measured by fluorescence-activated cell sorting. (C) Platelets were stimulated with 1 µg/mL CRP-XL for 3 minutes at 37°C. Phosphorylation of β3 (Y773) was measured by western blotting. Points represent mean responses relative to vehicle ± SEM. (D) Representative β3 (Y773) blots.

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