PU-H71 induces apoptosis in primary MCL cells. (A) Apoptosis measured by PI/annexin V double staining after 0.5 μM PU-H71 treatment in a representative tumor sample. (B) Line graphs showing increased apoptosis in 10 MCL tumor samples after 24 to 48 hours of PU-H71 treatment at 0.5 μM. (C) Line graph showing decreased cell viability in 6 additional MCL tumor samples after 24 and 48 hours of PU-H71 treatment. Student t test was used for statistical analysis for panels B-C. (D) Immunoblot showing reduced MYC expression after PU-H71 treatment for 24 hours in the 6 samples from panel C. (E) MYC protein levels determined by immunoblotting in panel D were quantified and normalized to GAPDH.