Specific activation of Nrf2 in monocytes/granulocytes alleviates inflammatory damage. (A) hematoxylin and eosin (H&E), F4/80, and chloroacetate esterase (Leder staining) staining of lungs from SCD::Keap1F/F (i,iii,v) mice and SCD::Keap1F/F::LysM-Cre (ii,iv,vi) mice showing congestion of the lungs and infiltration of inflammatory cells (i-ii), specifically macrophages (iii-iv) and neutrophils (v-vi). Black arrows show myeloid cells; arrowheads indicate macrophages, and red arrows denote neutrophils. Scale bar, 100 µm. (B) mRNA expression levels of tumor necrosis factor-α (TNF-α; left) and interleukin-1β (IL-1β; right) in the lungs of SCD::Keap1F/F (n = 6) and SCD::Keap1F/F::LysM-Cre mice (n = 6). (C) WBC counts in the peripheral blood of SCD::Keap1F/F (n = 7) and SCD::Keap1 F/F::LysM-Cre mice (n = 8). (D) Masson trichrome staining of livers from SCD::Keap1F/F (i,iii) and SCD::Keap1 F/F::LysM-Cre mice (ii,iv). Necrotic areas are delimited by dotted white lines. The areas in yellow squares are enlarged in panels iii-iv. (E) Quantification of necrotic areas in the liver. (F) Liver ALT levels of SCD::Keap1F/F (n = 9) and SCD::Keap1F/F::LysM-Cre mice (n = 11). The bar graphs represent the mean ± standard deviation. *P < .05; ***P < .001.