Keap1 deletion in monocytes/granulocytes improves heme and iron metabolism. (A) iMScope analysis of heme in SCD::Keap1F/F (i,iii) and SCD::Keap1F/F::LysM-Cre mouse livers (ii,iv). Bright-field images are shown in the upper panel (i-ii), and the yellow and red regions in the lower panel (iii-iv) represent heme accumulation in liver vessels and parenchyma. (B) Quantification of heme ion emissions in the vessels of the liver (upper) and parenchyma (lower) of SCD::Keap1F/F mice and SCD::Keap1F/F::LysM-Cre mice. (C) Plasma heme levels (left), plasma total bilirubin levels (middle), and plasma indirect bilirubin levels (right) of SCD::Keap1F/F mice and SCD::Keap1F/F LysM-Cre mice. (D) Prussian blue staining of iron in the livers (a,c) and kidneys (b,d) of SCD::Keap1F/F mice (a-b) and SCD::Keap1F/F::LysM-Cre mice (c-d). The red arrows show iron accumulation. Scale bar, 50 μm. (E) Matrix-assisted laser desorption/ionization iMScope of GSH (upper; i-ii) and GSSG (lower; iii-iv) in livers from SCD::Keap1F/F mice and SCD::Keap1F/F::LysM-Cre mice via iMScope. *P < .05; **P < .01.