EC-specific deletion of Keap1 rescues inflammation and liver function. (A) WBC counts in the peripheral blood of SCD::Keap1F/F mice (n = 10) and SCD::Keap1F/F::Tie1-Cre mice (n = 9). (B) H&E, F4/80, and chloroacetate esterase (Leder staining) staining of lungs from SCD::Keap1F/F (i,iii,v) mice and SCD::Keap1F/F::Tie1-Cre (ii,iv,vi) mice showing infiltration of macrophages (iii-iv) and neutrophils (v-vi) and congestion of the lungs of SCD::Keap1 F/F mice. Black arrows show myeloid cells and neutrophils; arrowheads indicate macrophages, and red arrows denote neutrophils. (C) Quantification of the mRNA levels of TNF-α (upper) and IL-1β (lower) in the lungs of SCD::Keap1F/F mice (n = 5) and SCD::Keap1F/F::Tie1-Cre mice (n = 6). (D) Masson trichrome staining of livers from SCD::Keap1F/F mice (i,iii) and SCD::Keap1F/F::Tie1-Cre mice (ii,iv). Scale bar, 100 µm. (E) Quantification analysis of necrotic areas in the liver. Necrotic areas were measured in each mouse using BZ Analyzer Software (KEYENCE) and are expressed as the percent of the total area. (F) Liver ALT levels of SCD::Keap1F/F mice (n = 5) and SCD::Keap1F/F::Tie1-Cre mice (n = 7). (G) Quantification of the mRNA levels of Hp, Ftl1, and Hamp in the livers of SCD::Keap1F/F mice (n = 5) and SCD::Keap1F/F::Tie1-Cre mice (n = 5). The bar graphs represent the mean ± standard deviation. *P < .05; **P < .01; ***P < .001.