Nrf2 activation in the ECs of SCD mice alters vascular leakage in vivo. (A) Quantification of the mRNA levels of Vcam1 (left) and P-selectin (right) in the lungs of SCD::Keap1F/F mice (n = 8) and SCD::Keap1F/F::Tie1-Cre mice (n = 8). (B) Schematic illustration of the Evans blue (EB) experiment. (C) Nrf2 activation in SCD::Keap1F/F::Tie1-Cre mice abrogates vascular leakage in the back skin. (D) Quantification of the ratio of formamide-extracted EB by spectrophotometry (610 nm), corrected for dry weight (DW) and hemoglobin (450 nm), in the livers and lungs of SCD::Keap1F/F and SCD::Keap1F/F::Tie1-Cre mice. *P < .05 (n = 5). OD, optical density.