Keap1 deletion in ECs upregulates the cell defense mechanism related to Nrf2. (A) Model of MPPEC culture and analysis. (B) Relative mRNA levels of Nqo1 in the MPPECs of SCD::Keap1F/F mice (n = 5) and SCD::Keap1F/F::Tie1-Cre mice (n = 5). *P < .05. (C) Scatter plots comparing transcript levels in SCD::Keap1F/F (x-axis) and SCD::Keap1F/F::Tie1-Cre (y-axis) MPPECs. A total of 879 (purple) genes were significantly upregulated, and 898 (mustard) genes were significantly downregulated in SCD Keap1F/F::Tie1-Cre MPPECs compared with those in SCD::Keap1F/F MPPECs. (D) Heat map showing relative expression levels in SCD::Keap1F/F (left) and SCD::Keap1F/F::Tie1-Cre mice (right). (E) Expression levels of Hmox1, Nos3, Vegfa, Vegfb, Gclm, and Gclc as fragments per kilobyte of exons per million fragments (FPKM) read values. TXN, thioredoxin.