ATF4 specifically binds to the BCL11A +55 enhancer. (A) Venn diagrams of the ATF4 ChIP-seq peaks in HUDEP2 and primary erythroblasts and the most enriched ATF4 binding motif in primary erythroblasts. (B) Heat maps of high-confidence ATF4 peaks in HUDEP2, BCL11A +55 sgRNA-treated, and primary erythroblasts. (C) Representative ATF4 ChIP-seq track of the BCL11A locus. The erythroid-specific enhancers +55 and +58 are shaded in purple. H3K27ac and DNaseI were obtained from published data.⁴¹  (D) ATF4 ChIP binding profile at the BCL11A +55 enhancer. The sgRNA targeting the ATF4 consensus motif is underlined, and the PAM sequence is shown in bold. (E) Genome-wide coverage of high-confident ATF4 peaks in HUDEP2 wild-type (WT) and BCL11A +55 sgRNA-treated cells. The BCL11A +55 peak is highlighted in red. RPM, reads per million.