Transplantability (self-renewal potential) of Lmo2-tg thymocytes is lost following deletion of Ldb1. (A) Schematic of experimental strategy. (B) Left panels, FACS analysis of total thymocytes from recipient B6 (CD45.1) mice that had been sublethally irradiated and injected intravenously with thymocytes from an Ldb1fl/fl;Lmo2-tg (CD45.2) donor mouse (upper panel) or a Rag1-Cre;Ldb1fl/fl;Lmo2-tg (CD45.2) donor mouse (lower panel) 8 weeks before analysis. Right panels, CD45.1 vs CD45.2 staining of total (live gate) or gated DN, DP, CD4 single positive (CD4SP), or CD8SP thymocytes from the indicated recipient mice. Numbers are percentage of donor (CD45.2) thymocytes in each group. One representative of 3 experiments. (C) Percent thymocyte chimerism (total thymocytes, top; DN thymocytes, bottom) in recipient mice 8 weeks after thymocyte transfer. (D) Kaplan-Meier survival plot of recipient mice (n = 3 mice of each genotype).