Upregulation of TPO in the acute platelet-depletion model. (A) Liver TPO mRNA was quantified by real-time quantitative PCR before and at the indicated time points after IV 5A7 injection. (B-C) Plasma TPO was measured by enzyme-linked immunosorbent assay before (B) and after (C) antibody injection, as indicated. ***P < .001 by 1-way analysis of variance, with Dunn’s multiple-comparison test.