Loss of miR-146a alters quiescence, myeloid differentiation, and primitiveness of HSCs. (A) Strategy for determining quiescence, proliferation, and differentiation of single ESLAMs. (B) Single-ESLAM proliferation kinetics of WT vs miR-146a^−/− (n = 97; 101 cells). (C) Percentage of single ESLAMs divided at 24 hours in culture, expressed as mean (±SEM) fold change relative to WT (n = 6; 5 replicates; P by Student t test). (D) Number of live cells produced from single ESLAMs after 10 days in culture (n = 34; 32 ESLAMs; P by Student t test). (E) Number of myeloid progenitor-like LK cells produced per single ESLAM clone shown in panel D (P by Student t test). (F-H) Pearson correlation coefficients and P-values (*P < .05; **P < .01, ***P < .001) for index sorting parameters vs (F) first cell division timing (Div.timing), determined in panel B; (G) clone size, determined in panel D; and (H) LK cell count (LK.count) per ESLAM clone, determined in panel E. (I) Relationship of EPCR^hi/CD150^hi ESLAM subpopulation (dashed boxes) to cell division timing (left), clone size, and LK cell count per clone (right), determined in panels B-E.