Loss of Setd2 promotes self-renewal of HSCs in the NHD13 mouse model. (A) Representative flow cytometry profiles of the Lin⁻, LK, and LSK cells in the BM of the transplant-recipient mice (left) and the primary BM cells (right) of the NHD13/Setd2^f/f or Mx1-Cre/NHD13/Setd2^f/f mice at 4 weeks after poly(I:C) injection. (B-C) Quantification of the numbers (B) and frequencies (C) of the Lin⁻, LK, and LSK cells in the BM of the transplant-recipient mice. (D-E) Representative flow cytometry profiles (D) and quantification of the frequencies (E) of the MPPs, LT-HSCs, and ST-HSCs of the NHD13/Setd2^f/f and NHD13/Setd2^Δ/Δ mice at 4 weeks after poly(I:C) injection. (F) The paired daughter cell assay analysis of the LSK cells isolated from BM of the NHD13/Setd2^f/f or NHD13/Setd2^Δ/Δ mice at 4 weeks after poly(I:C) injection. *P < .05; **P < .01; ***P < .001; ****P < .0001. AS, asymmetric self-renewal division; SD, symmetric commitment division; SS, symmetric self-renewal division.