Figure 1.
Abnormal in vitro T-cell differentiation of PB-CD34+cells with intrinsic deficiency. (A) A total of 1500 CD34+ cells from PB of an IL2RG/γc SCID patient and a control (cord blood) were differentiated in 3D culture for 3 weeks. (B) A total of 500 CD34+ cells from PB of a RAG (complete deficiency) SCID patient and control (cord blood) were differentiated in 3D culture for 5 weeks. (C) A total of 600 CD34+ cells from PB of a RAG (partial deficiency; Omenn syndrome) SCID patient and control (PB of healthy adult donor) were differentiated in 3D culture for 5 weeks. Displayed events are gated from single cells, negative for 7-AAD and positive for human CD45. Percentages among CD45+7-AAD−CD34−CD19− cells (left column) and CD45+7-AAD− cells (2 right columns) are indicated in inlets. FSC-A, forward scatter area.

Abnormal in vitro T-cell differentiation of PB-CD34+cells with intrinsic deficiency. (A) A total of 1500 CD34+ cells from PB of an IL2RG/γc SCID patient and a control (cord blood) were differentiated in 3D culture for 3 weeks. (B) A total of 500 CD34+ cells from PB of a RAG (complete deficiency) SCID patient and control (cord blood) were differentiated in 3D culture for 5 weeks. (C) A total of 600 CD34+ cells from PB of a RAG (partial deficiency; Omenn syndrome) SCID patient and control (PB of healthy adult donor) were differentiated in 3D culture for 5 weeks. Displayed events are gated from single cells, negative for 7-AAD and positive for human CD45. Percentages among CD45+7-AAD−CD34−CD19− cells (left column) and CD45+7-AAD− cells (2 right columns) are indicated in inlets. FSC-A, forward scatter area.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal