Figure 2.
Normal in vitro T-cell differentiation of PB-CD34+cells from SCID patients with thymic defects and ADA deficiency. (A) A total of 5000 CD34+ cells from PB of a TBX1 SCID patient and control (cord blood) were differentiated in 3D culture for 5 weeks. (B) A total of 1900 CD34+ cells from PB of a DiGeorge syndrome SCID patient and control (PB of a healthy adult donor) were differentiated in 3D culture for 5 weeks. (C) A total of 2000 CD34+ cells from PB of an ADA SCID patient and control (PB of a healthy adult donor) were differentiated in 3D culture for 3 weeks. Displayed events are gated from single cells, negative for 7-AAD and positive for human CD45. Percentages among CD45+7-AAD−CD34−CD19− cells (left column) and CD45+7-AAD− cells (2 right columns) are indicated in inlets.

Normal in vitro T-cell differentiation of PB-CD34+cells from SCID patients with thymic defects and ADA deficiency. (A) A total of 5000 CD34+ cells from PB of a TBX1 SCID patient and control (cord blood) were differentiated in 3D culture for 5 weeks. (B) A total of 1900 CD34+ cells from PB of a DiGeorge syndrome SCID patient and control (PB of a healthy adult donor) were differentiated in 3D culture for 5 weeks. (C) A total of 2000 CD34+ cells from PB of an ADA SCID patient and control (PB of a healthy adult donor) were differentiated in 3D culture for 3 weeks. Displayed events are gated from single cells, negative for 7-AAD and positive for human CD45. Percentages among CD45+7-AAD−CD34−CD19− cells (left column) and CD45+7-AAD− cells (2 right columns) are indicated in inlets.

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