The expression of recombinant TMs. (A) SDS-PAGE of recombinant TMs. Each protein (5 μg) reduced with 2-mercaptoethnol in the presence of SDS was loaded in each lane, and after electrophoresis, the proteins were stained with CBB. Lane 1, rhsTM G412D; lane 2, rhsTM; and lane 3, Recomodulin. (B) Western blotting of recombinant TMs. Each protein (4 μg) reduced with 2-mercaptoethnol in the presence of SDS was loaded in each lane, and after SDS-PAGE the proteins transferred onto the nitrocellulose membrane were detected with antihuman TM polyclonal antibody as described in the text. Lane 1, rhsTM G412D; lane 2, rhsTM; and lane 3, Recomodulin. The molecular weight of recombinant TMs was determined by a comparison with the mobility of commercial marker proteins.