The effects of recombinant TMs on plasma clotting time. The effects of rhsTM and rhsTM G412D on PT (A,D), APTT (B,E), and TCT (C,F) of human plasma were assessed by adding rhsTM (A-C) or rhsTM G412D (D-F), respectively. The detailed experimental conditions are described in the text. The results are expressed as the mean clotting time (sec) ± standard deviation of 3 independent experiments. Significant differences in values between samples with and without recombinant TM are shown as *P < .01.