The effects of recombinant TMs on the thrombin generation. The effects of various concentrations of rhsTM (A) and rhsTM G412D (B) on the TGT were determined using human plasma and a calibrated automated thrombogram system. The effect of rhsTM (blue triangles), rhsTM G412D (green triangles), or Recomodulin (red circles) on thrombin-catalyzed protein C activation (C) and TAFI activation (D) were determined using purified protein C and TAFI, respectively. (E) The thrombin-binding ability of rhsTM (blue triangles) and rhsTM G412D (green triangles) was determined by measuring the amidolytic activity of thrombin bound to recombinant TMs fixed in the microplate wells. The detailed experimental conditions are described in the text.