Dysbiosis does not affect homeostatic splenic cytokine levels before FVIII challenge. Spleens were isolated before FVIII challenge in ampicillin-treated (n = 3) and control (n = 4) mice. The protein content of splenic lysate was normalized and then analyzed on an 18-plex cytokine array (A) in addition to a TGF-β panel (B). The means and standard deviations in the cohorts were compared with the unpaired, 2-tailed Student t test. Multiple test corrections were made by the Holm-Šidák method.