Figure 1.
Characterization of TER119 (rat IgG2b) in vitro and in vivo. (A) To assess anemia, mice were uninjected (□—□), injected with 40 µg isotype control antibody (◆—◆), or injected with 40 µg TER119 antibody (●–●). Mice were bled and erythrocytes enumerated at the indicated time points. Day 0 counts represent the erythrocyte count before treatment; n = 5-8. Statistical significance was determined using a 2-way analysis of variance followed by Tukey’s test. (B) To test for therapeutic efficacy in ITP, mice were injected with the antierythrocyte antibody followed by MWReg30 (antiplatelet antibody) after 30 minutes to induce thrombocytopenia. Platelet counts were assessed 24 hours later. “Nil” represents the baseline platelet count of the control untreated mice, while ITP refers to the mice receiving the antiplatelet antibody alone (with no therapeutic treatment); n = 6-7. Statistical significance was determined using the Mann-Whitney U test. (C-E) Erythrocyte phagocytosis. Erythrocytes were sensitized with the isotype control antibody (C) or TER119 antibody (D) and incubated with RAW 264.7 macrophages to assess phagocytosis. Phagocytosis was observed using an inverted light microscope. Engulfed erythrocytes (D; arrow points to an engulfed erythrocyte) are seen as a red-brown sphere within macrophages. Phagocytosis was quantified using the erythrocyte phagocytic index (ePI; E); n = 4-8. Statistical significance was determined using the Mann-Whitney U test. (F-H) Inhibition of platelet phagocytosis. Platelets were labeled with a cell tracker dye (green) and sensitized with MWReg30 (antiplatelet antibody). Erythrocytes were sensitized with the isotype control antibody (F) or TER119 antibody (G). Sensitized platelets and erythrocytes were simultaneously incubated with RAW 264.7 macrophages. Platelet phagocytosis was assessed by confocal microscopy. Engulfed platelets (F; arrow points to an engulfed platelet) are seen as a green sphere within macrophages. Inhibition of platelet phagocytosis (H) was calculated relative to the sensitized platelets group for that experiment (no treatment; arbitrarily set as 0% inhibition); n = 3-6. Statistical significance was determined using the Mann-Whitney U test. ** P < .01, **** P < .0001 (ns, not significant).

Characterization of TER119 (rat IgG2b) in vitro and in vivo. (A) To assess anemia, mice were uninjected (□—□), injected with 40 µg isotype control antibody (◆—◆), or injected with 40 µg TER119 antibody (●–●). Mice were bled and erythrocytes enumerated at the indicated time points. Day 0 counts represent the erythrocyte count before treatment; n = 5-8. Statistical significance was determined using a 2-way analysis of variance followed by Tukey’s test. (B) To test for therapeutic efficacy in ITP, mice were injected with the antierythrocyte antibody followed by MWReg30 (antiplatelet antibody) after 30 minutes to induce thrombocytopenia. Platelet counts were assessed 24 hours later. “Nil” represents the baseline platelet count of the control untreated mice, while ITP refers to the mice receiving the antiplatelet antibody alone (with no therapeutic treatment); n = 6-7. Statistical significance was determined using the Mann-Whitney U test. (C-E) Erythrocyte phagocytosis. Erythrocytes were sensitized with the isotype control antibody (C) or TER119 antibody (D) and incubated with RAW 264.7 macrophages to assess phagocytosis. Phagocytosis was observed using an inverted light microscope. Engulfed erythrocytes (D; arrow points to an engulfed erythrocyte) are seen as a red-brown sphere within macrophages. Phagocytosis was quantified using the erythrocyte phagocytic index (ePI; E); n = 4-8. Statistical significance was determined using the Mann-Whitney U test. (F-H) Inhibition of platelet phagocytosis. Platelets were labeled with a cell tracker dye (green) and sensitized with MWReg30 (antiplatelet antibody). Erythrocytes were sensitized with the isotype control antibody (F) or TER119 antibody (G). Sensitized platelets and erythrocytes were simultaneously incubated with RAW 264.7 macrophages. Platelet phagocytosis was assessed by confocal microscopy. Engulfed platelets (F; arrow points to an engulfed platelet) are seen as a green sphere within macrophages. Inhibition of platelet phagocytosis (H) was calculated relative to the sensitized platelets group for that experiment (no treatment; arbitrarily set as 0% inhibition); n = 3-6. Statistical significance was determined using the Mann-Whitney U test. ** P < .01, **** P < .0001 (ns, not significant).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal