SPADE plots depicting population relationships during in vitro differentiation. (A) Plots showing related phenotypes in healthy and WM samples based on CD38 and CD138 expression among day-13 cells that had been stimulated with CD40L and F(ab′)₂ anti-IgG/IgM at day 0. Each node is colored according to the median intensity of the indicated marker and is proportional in size to the number of cells with that marker intensity. (B) Visualization of the branched interconnectivity of healthy day-13 cells vs all LPDs, including WM, in response to CD40L or R848 stimulation based on CD38 or CD138 expression.