Clonal evolution patterns of regression and selection in rrDLBCL. (A) Number of cases with a mutation that regressed (blue), expanded (pink), or remained stable (gray) in a given gene following therapy. Time points are from a tumor biopsy before treatment (T1) and a plasma sample after treatment (P2). Genes in clusters that predominately undergo clonal expansion treatment are near the bottom, and genes in clusters depleted following treatment are near the top. (B-I) Clonal evolution plots for several patients following therapy, using a pretreatment tumor tissue biopsy and a posttreatment plasma sample. Each line represents a single coding mutation, and the relative CCF of each mutation before and after therapy is used to flag mutations that undergo clonal expansion (pink), depletion (blue), or remain stable (gray). The 8 genes differentially mutated are labeled and highlighted, and the mutation type is indicated by the adjacent symbol.