Impaired migration and proliferation of peripheral blood lymphocytes. (A) Percentage of migrated freshly isolated lymphocytes after 3 hours of stimulation with increasing concentrations of SDF1-α in P1 and P2 as compared with HC (n = 6). Mean ± SEM. (B) Spontaneous migration in P1 (red bar), P2 (blue bar), and HC (n = 6; green bar). Mean ± SEM. (C-D) Percentage of proliferation determined by cell trace dilution in CD4⁺ and CD8⁺ T cells for HC (blue, n = 3) and patients P1, P2, and P6 with anti-CD3 alone or in combination with anti-CD28. Median (bar) and patients’ symbols are indicated. Stimulation index: ratio between stimulated and unstimulated cells. (E) Restoration of proliferation in transduced PHA T-cell blasts of P2 and a HC. T cells were stimulated with increasing doses of plate-bound anti-CD3. Graph summarizes the proliferation of T cells sorted according to mCherry or GFP expression. Vectors used for transduction are indicated. Mean ± SEM. cpm, counts per minute.