Analysis of BCL2 family members’ expression and cell dependencies in MM patient samples. (A) Analysis of BCL2/BCLXL, BCL2/MCL1, and BCL2/(MCL1+BCLXL) mRNA expression ratio in function of venetoclax sensitivity in patients with myeloma (n = 41). BCL2, BCLXL and MCL1 mRNA levels were defined by quantitative polymerase chain reaction, using Taq-Man probes (supplemental Table 2), and the different ratios were plotted against venetoclax sensitivity defined by the principal component (PC1) values (supplemental Figure 4A). The Spearman rank correlation is indicated. (B) Analysis of MCL1/BCLXL, MCL1/BCL2, and MCL1/BCLXL+BCL2 mRNA expression ratio in function of A1210477 sensitivity defined by the principal component (PC1) values in patients with myeloma (n = 41). Correlation was assessed by Spearman test. (C) Comparison of dependencies between HMCLs (n = 33) and patients at relapse (n = 39). Sensitivity to each BH3 mimetic of HMCLs is provided in Table 2.