CiTE and sctb enhance lysis of CD33⁺PD-L1⁺target cells. (A) Dose-dependent lysis of MOLM-13 vs MOLM-13:PD-L1 cells or (B) OCI-AML3 vs OCI-AML3:PD-L1 cells by nonstimulated HD T cells. Calculated EC₅₀ values for CiTE and sctb on MOLM-13 cells were 79.1 and 81.6 pM, on MOLM-13:PD-L1 cells were 26.9 and 13.2 pM, on OCI-AML3 cells were 20.8 and 27.4 pM, and on OCI-AML3:PD-L1 cells were 2.3 and 1.9 pM, respectively. (C) Lysis of MOLM-13 vs MOLM-13:PD-L1 cells at 50 pM protein concentration and (D) OCI-AML3 vs OCI-AML3:PD-L1 cells at 5 pM concentration at an E:T ratio of 2:1 after 72 hours. Lysis of (E) MOLM-13:PD-L1 cells and (F) BA/F3:CD33:PD-L1 cells at 0.5 nM concentration, E:T ratio of 1:3, and culture time of 72 hours. Graphs display mean values of (A-D) n = 4-5, (E) n = 7, or (F) n = 6 independent experiments with SEM as error bars. For statistical analysis, unpaired Student t test with Welch correction (C-D) or Wilcoxon test (E-F) was applied. *P < .05, **P < .01, ***P < .001.