CiTE induces preferential lysis of CD33⁺PD-L1⁺cells and has no activity against CD33^-PD-L1⁺cells. Dose-dependent preferential lysis of (A) HEK:CD33:PD-L1 over (B) HEK:PD-L1 cells induced by CiTE, sctb, or BiTE-like molecule and vice versa. Preactivated HD T cells were incubated with target cells and CiTE, sctb, or BiTE-like molecule for 4 hours at a total E:T ratio of 2:1. EC₅₀ values for CiTE, sctb, and BiTE-like molecule were 22.8, 10.4, and 13.5 pM for HEK:CD33:PD-L1 cells. For HEK:PD-L1 cells, the EC₅₀ value for the sctb was 114.4 pM. Graphs represent mean values of n = 4 independent experiments with SEM as error bars.